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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RNASEH2B Recombinant antibody, PBS Only

RNASEH2B Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241131F1

Cat no : 84097-6-PBS

Synonyms

ribonuclease H2, subunit B, Ribonuclease H2 subunit B, DLEU8, Aicardi-Goutieres syndrome 2 protein, AGS2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNASEH2B Recombinant antibody (84097-6-RR)

    WB analysis using 84097-6-RR (same clone as 84097-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84097-6-RR (RNASEH2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84097-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RNASEH2B Recombinant antibody (84097-6-RR)

    IF Staining of HeLa using 84097-6-RR (same clone as 84097-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RNASEH2B antibody (84097-6-RR, Clone: 241131F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84097-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84097-6-RR

    Affinity and Kinetic Characterization of 84097-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84067-4-RR against Human RNASEH2B were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 84097-6-PBS is RNASEH2B Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RNASEH2B fusion protein Ag33669 相同性解析による交差性が予測される生物種
Full Name ribonuclease H2, subunit B
Calculated molecular weight 331 aa, 37 kDa
Observed molecular weight35 kDa
GenBank accession numberBC036744
Gene symbol RNASEH2B
Gene ID (NCBI) 79621
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Ribonuclease H2 is the major nuclear enzyme degrading cellular RNA/DNA hybrids in eukaryotes and the sole nuclease known to be able to hydrolyze ribonucleotides misincorporated during genomic replication. RNASEH2B and RNASEH2C are the non-catalytic subunits of RNase H2 complex. Defects in RNASEH2B are a cause of Aicardi-Goutieres syndrome type 2 (AGS2), a genetically heterogeneous disease with clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process.