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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RNASEH2B Recombinant antibody

RNASEH2B Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241131F1

Cat no : 84097-6-RR

Synonyms

ribonuclease H2, subunit B, Ribonuclease H2 subunit B, DLEU8, Aicardi-Goutieres syndrome 2 protein, AGS2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNASEH2B Recombinant antibody (84097-6-RR)

    WB analysis using 84097-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84097-6-RR (RNASEH2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RNASEH2B Recombinant antibody (84097-6-RR)

    IF Staining of HeLa using 84097-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RNASEH2B antibody (84097-6-RR, Clone: 241131F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84097-6-RR

    Affinity and Kinetic Characterization of 84097-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84067-4-RR against Human RNASEH2B were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, Jurkat cells, U-937 cells, THP-1 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84097-6-RR is RNASEH2B Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RNASEH2B fusion protein Ag33669 相同性解析による交差性が予測される生物種
Full Name ribonuclease H2, subunit B
Calculated molecular weight 331 aa, 37 kDa
Observed molecular weight35 kDa
GenBank accession numberBC036744
Gene symbol RNASEH2B
Gene ID (NCBI) 79621
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribonuclease H2 is the major nuclear enzyme degrading cellular RNA/DNA hybrids in eukaryotes and the sole nuclease known to be able to hydrolyze ribonucleotides misincorporated during genomic replication. RNASEH2B and RNASEH2C are the non-catalytic subunits of RNase H2 complex. Defects in RNASEH2B are a cause of Aicardi-Goutieres syndrome type 2 (AGS2), a genetically heterogeneous disease with clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process.

Protocols

Product Specific Protocols
WB protocol for RNASEH2B antibody 84097-6-RRDownload protocol
IF protocol for RNASEH2B antibody 84097-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols