• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using RNF2 Recombinant antibody (84793-5-RR)

    WB analysis using 84793-5-RR (same clone as 84793-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84793-5-RR (RNF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84793-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using RNF2 Recombinant antibody (84793-5-RR)

    WB analysis of HEK-293 using 84793-5-RR (same clone as 84793-5-PBS)

    WB result of RNF2 antibody (84793-5-RR; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNF2 transfected HEK-293 cells. This data was developed using the same antibody clone with 84793-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using RNF2 Recombinant antibody (84793-5-RR)

    IF Staining of U2OS using 84793-5-RR (same clone as 84793-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RNF2 antibody (84793-5-RR, Clone: 242181G7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84793-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using RNF2 Recombinant antibody (84793-5-RR)

    FC experiment of HepG2 using 84793-5-RR (same clone as 84793-5-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RNF2 Recombinant antibody (84793-5-RR, Clone:242181G7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84793-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84793-5-RR

    Affinity and Kinetic Characterization of 84793-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84793-5-RR against Human RNF2 were performed. The affinity constant is 0.251 nM.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

  • Featured Product
  • KD/KO Validated

RNF2 Recombinant antibody, PBS Only

RNF2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84793-5-PBS
Clone No.242181G7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

EC:2.3.2.27, E3 ubiquitin-protein ligase RING2, DING, BAP1, BAP 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84793-5-PBS targets RNF2 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RNF2 fusion protein Ag8900 相同性解析による交差性が予測される生物種
Full Name ring finger protein 2
Calculated molecular weight 336 aa, 38 kDa
Observed molecular weight38 kDa
GenBank accession numberBC012583
Gene Symbol RNF2
Gene ID (NCBI) 6045
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ99496
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

RNF2 (also known as ding, Ring1B or Ring2) is a member of the Ring finger protein family, which functions as E3 ubiquitin ligase for monoubiquitination of histone H2A at lysine 119 (H2AK119ub). Many studies have demonstrated that overexpressed RNF2 was involved in the pathological progression of multiple cancers and has an impact on their clinical features. For instance, the upregulated expression level of RNF2 is positively correlated with the occurrence and progression of hepatocellular carcinoma, melanoma, prostate cancer, breast cancer, pancreatic cancer, gastric cancer, and bladder urothelial carcinoma, as well as with the radioresistance of lung cancer and chemoresistance of ovarian cancer (PMID: 33400276). The molecular weight of RNF2 is 38 kDa.