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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RNF2 Recombinant antibody

RNF2 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

242181G7

Cat no : 84793-5-RR

Synonyms

EC:2.3.2.27, E3 ubiquitin-protein ligase RING2, DING, BAP1, BAP 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNF2 Recombinant antibody (84793-5-RR)

    WB analysis using 84793-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84793-5-RR (RNF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RNF2 Recombinant antibody (84793-5-RR)

    WB analysis of HEK-293 using 84793-5-RR

    WB result of RNF2 antibody (84793-5-RR; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNF2 transfected HEK-293 cells.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using RNF2 Recombinant antibody (84793-5-RR)

    IF Staining of U2OS using 84793-5-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RNF2 antibody (84793-5-RR, Clone: 242181G7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using RNF2 Recombinant antibody (84793-5-RR)

    FC experiment of HepG2 using 84793-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RNF2 Recombinant antibody (84793-5-RR, Clone:242181G7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84793-5-RR

    Affinity and Kinetic Characterization of 84793-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84793-5-RR against Human RNF2 were performed. The affinity constant is 0.251 nM.

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Tested Applications

Positive WB detected inJurkat cells, K-562 cells, HeLa cells, RAW 264.7 cells, NIH/3T3 cells
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84793-5-RR targets RNF2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RNF2 fusion protein Ag8900 相同性解析による交差性が予測される生物種
Full Name ring finger protein 2
Calculated molecular weight 336 aa, 38 kDa
Observed molecular weight38 kDa
GenBank accession numberBC012583
Gene symbol RNF2
Gene ID (NCBI) 6045
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RNF2 (also known as ding, Ring1B or Ring2) is a member of the Ring finger protein family, which functions as E3 ubiquitin ligase for monoubiquitination of histone H2A at lysine 119 (H2AK119ub). Many studies have demonstrated that overexpressed RNF2 was involved in the pathological progression of multiple cancers and has an impact on their clinical features. For instance, the upregulated expression level of RNF2 is positively correlated with the occurrence and progression of hepatocellular carcinoma, melanoma, prostate cancer, breast cancer, pancreatic cancer, gastric cancer, and bladder urothelial carcinoma, as well as with the radioresistance of lung cancer and chemoresistance of ovarian cancer (PMID: 33400276). The molecular weight of RNF2 is 38 kDa.

Protocols

Product Specific Protocols
WB protocol for RNF2 antibody 84793-5-RRDownload protocol
IF protocol for RNF2 antibody 84793-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols