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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human liver tissue using RNF217 Polyclonal antibody (26084-1-AP)

    IHC staining of human liver using 26084-1-AP (same clone as 26084-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26084-1-AP (RNF217 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26084-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using RNF217 Polyclonal antibody (26084-1-AP)

    IHC staining of human liver using 26084-1-AP (same clone as 26084-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26084-1-AP (RNF217 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26084-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RNF217 Polyclonal antibody (26084-1-AP)

    IF Staining of HepG2 using 26084-1-AP (same clone as 26084-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RNF217 antibody (26084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26084-1-PBS in a different storage buffer formulation.

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RNF217 Polyclonal antibody, PBS Only

RNF217 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 26084-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26084-1-PBS targets RNF217 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF217 fusion protein Ag22022 相同性解析による交差性が予測される生物種
Full Name ring finger protein 217
Calculated molecular weight 275 aa, 32 kDa
Observed molecular weight 59, 32 kDa
GenBank accession numberBC026087
Gene Symbol RNF217
Gene ID (NCBI) 154214
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TC41
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

E3 ubiquitin-protein ligase RNF217, also known as IBR domain-containing protein 1 (IBRDC1), as a transmembrane (TM) domain-containing RBR-type E3 ubiquitin-protein ligase, belongs to a highly conserved member of the RING finger family. RNF217 mediates the degradation of the iron exporter ferroportin/SLC40A1 and thus regulates iron homeostasis(Uniprot, PMID: 33895792).