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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RNF31 / HOIP Recombinant antibody

RNF31 / HOIP Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230446H1

Cat no : 83137-3-RR

Synonyms

HOIP, RNF31, HOIL 1 interacting protein, FLJ23501, FLJ10111

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RNF31 / HOIP Recombinant antibody (83137-3-RR)

    IF Staining of HepG2 using 83137-3-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RNF31 antibody (83137-3-RR, Clone: 230446H1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of PC-3 cells using RNF31 / HOIP Recombinant antibody (83137-3-RR)

    FC experiment of PC-3 using 83137-3-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.2 ug Rnf31 Recombinant Antibody (83137-3-RR, Clone:230446H1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83137-3-RR

    Affinity and Kinetic Characterization of 83137-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83137-3-RR against Human RNF31 / HOIP were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inPC-3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83137-3-RR targets RNF31 / HOIP in IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Affinity
KD=5.82 x 10-10M
KOff=5.93 x 10-5M
KOn=1.02 x 105M
Immunogen RNF31 / HOIP fusion protein Ag34505 相同性解析による交差性が予測される生物種
Full Name ring finger protein 31
Calculated molecular weight 1072 aa, 120 kDa
GenBank accession numberBC017376
Gene symbol RNF31
Gene ID (NCBI) 55072
RRIDAB_3670838
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for RNF31 / HOIP antibody 83137-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols