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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RNF31 / HOIP Recombinant antibody

RNF31 / HOIP Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230446H1

Cat no : 83137-3-RR

Synonyms

HOIP, RNF31, HOIL 1 interacting protein, FLJ23501, FLJ10111

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RNF31 / HOIP Recombinant antibody (83137-3-RR)

    IF Staining of HepG2 using 83137-3-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RNF31 antibody (83137-3-RR, Clone: 230446H1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of PC-3 cells using RNF31 / HOIP Recombinant antibody (83137-3-RR)

    FC experiment of PC-3 using 83137-3-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.2 ug Rnf31 Recombinant Antibody (83137-3-RR, Clone:230446H1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83137-3-RR

    Affinity and Kinetic Characterization of 83137-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83137-3-RR against Human RNF31 / HOIP were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inPC-3 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83137-3-RR targets RNF31 / HOIP in IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Affinity
KD=5.82 x 10-10M
KOff=5.93 x 10-5M
KOn=1.02 x 105M
Immunogen RNF31 / HOIP fusion protein Ag34505 相同性解析による交差性が予測される生物種
Full Name ring finger protein 31
Calculated molecular weight 1072 aa, 120 kDa
GenBank accession numberBC017376
Gene symbol RNF31
Gene ID (NCBI) 55072
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for RNF31 / HOIP antibody 83137-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols