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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated RNF31 / HOIP Recombinant antibody

RNF31 / HOIP Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230446H1

Cat no : CL488-83137-3

Synonyms

HOIP, RNF31, HOIL 1 interacting protein, FLJ23501, FLJ10111

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated RNF31 / HOIP Recombi (CL488-83137-3)

    IF Staining of HepG2 using CL488-83137-3

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 RNF31 / HOIP antibody (CL488-83137-3, Clone: 230446H1 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated RNF31 / HOIP Recombi (CL488-83137-3)

    FC experiment of HeLa using CL488-83137-3

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Rnf31 / HOIP Recombinant Antibody (CL488-83137-3, Clone:230446H1)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83137-3 targets RNF31 / HOIP in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RNF31 / HOIP fusion protein Ag34505 相同性解析による交差性が予測される生物種
Full Name ring finger protein 31
Calculated molecular weight 1072 aa, 120 kDa
GenBank accession numberBC017376
Gene symbol RNF31
Gene ID (NCBI) 55072
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RNF31 / HOIP antibody CL488-83137-3Download protocol
FC protocol for CL Plus 488 RNF31 / HOIP antibody CL488-83137-3Download protocol
Standard Protocols
Click here to view our Standard Protocols