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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNGTT Recombinant antibody (83974-2-RR)

    WB analysis using 83974-2-RR (same clone as 83974-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83974-2-RR (RNGTT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83974-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using RNGTT Recombinant antibody (83974-2-RR)

    FC experiment of HeLa using 83974-2-RR (same clone as 83974-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RNGTT Recombinant antibody (83974-2-RR, Clone:240927A10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83974-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00899-2

    Cytometric bead array standard curve of MP00899-2

    Cytometric bead array standard curve of MP00899-2, RNGTT Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83974-2-PBS. Detection antibody: 83974-3-PBS. Standard: Ag3105. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 83974-2-RR

    Affinity and Kinetic Characterization of 83974-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83974-2-RR against Human RNGTT were performed. The affinity constant is below 1 pM.

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RNGTT Recombinant antibody, PBS Only (Capture)

RNGTT Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83974-2-PBS
Clone No.240927A10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

hCAP, GTP--RNA guanylyltransferase, GTP  RNA guanylyltransferase, GTase, CAP1A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83974-2-PBS targets RNGTT as part of a matched antibody pair:

MP00899-2: 83974-2-PBS capture and 83974-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RNGTT fusion protein Ag3105 相同性解析による交差性が予測される生物種
Full Name RNA guanylyltransferase and 5'-phosphatase
Calculated molecular weight 597 aa, 69 kDa
Observed molecular weight62-68 kDa
GenBank accession numberBC019954
Gene Symbol RNGTT
Gene ID (NCBI) 8732
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60942
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

RNGTT(mRNA-capping enzyme) is also named as HCAP1, HCE, CAP1A. In the N-terminal section it belongs to the non-receptor class of the protein-tyrosine phosphatase family and in the C-terminal section it belongs to the eukaryotic GTase family. It is a bifunctional 597-amino acid protein with RNA triphosphatase and RNA guanylyltransferase activities(PMID:9545288). It has 4 isoforms produced by alternative splicing.