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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNGTT Recombinant antibody (83974-2-RR)

    WB analysis using 83974-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83974-2-RR (RNGTT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using RNGTT Recombinant antibody (83974-2-RR)

    FC experiment of HeLa using 83974-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RNGTT Recombinant antibody (83974-2-RR, Clone:240927A10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83974-2-RR

    Affinity and Kinetic Characterization of 83974-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83974-2-RR against Human RNGTT were performed. The affinity constant is below 1 pM.

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RNGTT Recombinant antibody

RNGTT Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83974-2-RR
Clone No.240927A10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

hCAP, GTP--RNA guanylyltransferase, GTP  RNA guanylyltransferase, GTase, CAP1A

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, MCF-7 cells, K-562 cells, HEK-293T cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83974-2-RR targets RNGTT in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RNGTT fusion protein Ag3105 相同性解析による交差性が予測される生物種
Full Name RNA guanylyltransferase and 5'-phosphatase
Calculated molecular weight 597 aa, 69 kDa
Observed molecular weight62-68 kDa
GenBank accession numberBC019954
Gene Symbol RNGTT
Gene ID (NCBI) 8732
RRIDAB_3671549
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60942
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RNGTT(mRNA-capping enzyme) is also named as HCAP1, HCE, CAP1A. In the N-terminal section it belongs to the non-receptor class of the protein-tyrosine phosphatase family and in the C-terminal section it belongs to the eukaryotic GTase family. It is a bifunctional 597-amino acid protein with RNA triphosphatase and RNA guanylyltransferase activities(PMID:9545288). It has 4 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for RNGTT antibody 83974-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols