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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated RORA Recombinant ant (CL488-82930)

    IF Staining of HepG2 using CL488-82930

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 RORA antibody (CL488-82930, Clone: 230217G6 ) at dilution of 1:200.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CoraLite® Plus 488-conjugated RORA Recombinant antibody

RORA Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL488-82930
Clone No.230217G6

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC

Synonyms

Retinoid-related orphan receptor-alpha, RAR-related orphan receptor A, Nuclear receptor RZR-alpha, Nuclear receptor ROR-alpha, NR1F1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82930 targets RORA in IF/ICC applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RORA fusion protein Ag0944 相同性解析による交差性が予測される生物種
Full Name RAR-related orphan receptor A
Calculated molecular weight 59 kDa
Observed molecular weight60-66 kDa
GenBank accession numberBC008831
Gene Symbol RORA
Gene ID (NCBI) 6095
RRIDAB_3673140
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35398
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RORA is a steroid hormone receptor and has been implicated in the pathology of circadian rhythms, bone growth, angiogenesis, development of cones, cellular metabolism and a mediator in the immune and lipid metabolism pathways [PMID:21998696]. It binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence, which binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPAR gamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM and ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1 [PMID:11053433,14570920].

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RORA antibody CL488-82930Download protocol
Standard Protocols
Click here to view our Standard Protocols