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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated RPA2 Polyclonal anti (CL488-10412)

    IF Staining of A431 using CL488-10412

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 RPA2 antibody (CL488-10412) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of U2OS cells using CoraLite® Plus 488-conjugated RPA2 Polyclonal anti (CL488-10412)

    FC experiment of U2OS using CL488-10412

    1x10^6 U2OS cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Rpa2 Polyclonal Antibody (CL488-10412)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 488-conjugated RPA2 Polyclonal antibody

RPA2 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-10412

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

RP A p34, RP A p32, RF-A protein 2, replication protein A2, 32kDa, Replication factor A protein 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10412 targets RPA2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPA2 fusion protein Ag0690 相同性解析による交差性が予測される生物種
Full Name replication protein A2, 32kDa
Calculated molecular weight 29 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC001630
Gene Symbol RPA2
Gene ID (NCBI) 6118
RRIDAB_3672440
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15927
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. RPA2 is a major target for upstream kinases, including CDKs and the three PIKKs (ATR, ataxia-telangiectasia mutated [ATM], and DNA-PK). RPA2 comprises multiple critical Ser/Tr residues that are phosphorylated in sequential order in response to genotoxic stress.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RPA2 antibody CL488-10412Download protocol
Standard Protocols
Click here to view our Standard Protocols