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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated RPA2 Monoclonal antibody

RPA2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1B9H11

Cat no : CL594-67999

Synonyms

RP A p32, RF-A protein 2, replication protein A2, 32kDa, Replication factor A protein 2, REPA2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite®594-conjugated RPA2 Monoclonal antibody (CL594-67999)

    IF Staining of A431 using CL594-67999

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®594 RPA2 antibody (CL594-67999, Clone: 1B9H11 ) at dilution of 1:1000.

  • Flow cytometry (FC) experiment of A431 cells using CoraLite®594-conjugated RPA2 Monoclonal antibody (CL594-67999)

    FC experiment of A431 using CL594-67999

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RPA2 (CL594-67999, Clone:1B9H11) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67999 targets RPA2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RPA2 fusion protein Ag24783 相同性解析による交差性が予測される生物種
Full Name replication protein A2, 32kDa
Calculated molecular weight 29 kDa
Observed molecular weight30 kDa
GenBank accession numberBC001630
Gene symbol RPA2
Gene ID (NCBI) 6118
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. RPA2 is a major target for upstream kinases, including CDKs and the three PIKKs (ATR, ataxia-telangiectasia mutated [ATM], and DNA-PK). RPA2 comprises multiple critical Ser/Thr residues that are phosphorylated in sequential order in response to genotoxic stress.

Protocols

Product Specific Protocols
IF protocol for CL594 RPA2 antibody CL594-67999Download protocol
FC protocol for CL594 RPA2 antibody CL594-67999Download protocol
Standard Protocols
Click here to view our Standard Protocols