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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using RPAP3 Polyclonal antibody (23741-1-AP)

    WB analysis of HEK-293 using 23741-1-AP (same clone as 23741-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23741-1-AP (RPAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using RPAP3 Polyclonal antibody (23741-1-AP)

    WB analysis of Jurkat using 23741-1-AP (same clone as 23741-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 23741-1-AP (RPAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using RPAP3 Polyclonal antibody (23741-1-AP)

    WB analysis of HeLa using 23741-1-AP (same clone as 23741-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 23741-1-AP (RPAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using RPAP3 Polyclonal antibody (23741-1-AP)

    WB analysis of Jurkat using 23741-1-AP (same clone as 23741-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 23741-1-AP (RPAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using RPAP3 Polyclonal antibody (23741-1-AP)

    IP experiment of HEK-293 using 23741-1-AP (same clone as 23741-1-PBS)

    IP result of anti-RPAP3 (IP:23741-1-AP, 4ug; Detection:23741-1-AP 1:500) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RPAP3 Polyclonal antibody (23741-1-AP)

    IHC staining of human stomach cancer using 23741-1-AP (same clone as 23741-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23741-1-AP (RPAP3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RPAP3 Polyclonal antibody (23741-1-AP)

    IHC staining of human stomach cancer using 23741-1-AP (same clone as 23741-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23741-1-AP (RPAP3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RPAP3 Polyclonal antibody (23741-1-AP)

    IF Staining of HeLa using 23741-1-AP (same clone as 23741-1-PBS)

    Immunofluorescent analysis of HeLa cells using 23741-1-AP (RPAP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

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RPAP3 Polyclonal antibody, PBS Only

RPAP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 23741-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23741-1-PBS targets RPAP3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPAP3 fusion protein Ag20661 相同性解析による交差性が予測される生物種
Full Name RNA polymerase II associated protein 3
Calculated molecular weight 76 kDa
Observed molecular weight 76 kDa
GenBank accession numberBC056415
Gene Symbol RPAP3
Gene ID (NCBI) 79657
RRIDAB_2879313
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9H6T3
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.