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Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using RPH3AL Polyclonal antibody (15297-1-AP)

    WB analysis of human liver using 15297-1-AP (same clone as 15297-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 15297-1-AP (RPH3AL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15297-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using RPH3AL Polyclonal antibody (15297-1-AP)

    WB analysis of L02 using 15297-1-AP (same clone as 15297-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 15297-1-AP (RPH3AL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15297-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using RPH3AL Polyclonal antibody (15297-1-AP)

    IP experiment of HepG2 using 15297-1-AP (same clone as 15297-1-PBS)

    IP result of anti-RPH3AL (IP:15297-1-AP, 4ug; Detection:15297-1-AP 1:500) with HepG2 cells lysate 2400ug. This data was developed using the same antibody clone with 15297-1-PBS in a different storage buffer formulation.

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RPH3AL Polyclonal antibody, PBS Only

RPH3AL Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 15297-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15297-1-PBS targets RPH3AL in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPH3AL fusion protein Ag7552 相同性解析による交差性が予測される生物種
Full Name rabphilin 3A-like (without C2 domains)
Calculated molecular weight 34 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC005153
Gene Symbol RPH3AL
Gene ID (NCBI) 9501
RRIDAB_2181270
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UNE2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Rabphilin-3A-like (RPH3AL), also known as Noc2, is a Rab effector protein with a molecular weight of 38 kDa. It is expressed abundantly in endocrine cells but at low levels in exocrine tissues, playing a direct regulatory role in calcium-ion-dependent exocytosis in both endocrine and exocrine cells, and also having a key role in INS secretion by pancreatic cells. RPH3AL functions as a potential RAB3B effector protein in epithelial cells, and RPH3AL gene is likely a tumor suppressor.