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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPH3AL Polyclonal antibody, PBS Only

RPH3AL Polyclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15297-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using RPH3AL Polyclonal antibody (15297-1-AP)

    WB analysis of human liver using 15297-1-AP (same clone as 15297-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 15297-1-AP (RPH3AL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15297-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using RPH3AL Polyclonal antibody (15297-1-AP)

    WB analysis of L02 using 15297-1-AP (same clone as 15297-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 15297-1-AP (RPH3AL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15297-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using RPH3AL Polyclonal antibody (15297-1-AP)

    IP experiment of HepG2 using 15297-1-AP (same clone as 15297-1-PBS)

    IP result of anti-RPH3AL (IP:15297-1-AP, 4ug; Detection:15297-1-AP 1:500) with HepG2 cells lysate 2400ug. This data was developed using the same antibody clone with 15297-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15297-1-PBS targets RPH3AL in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPH3AL fusion protein Ag7552 相同性解析による交差性が予測される生物種
Full Name rabphilin 3A-like (without C2 domains)
Calculated molecular weight 34 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC005153
Gene symbol RPH3AL
Gene ID (NCBI) 9501
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Rabphilin-3A-like (RPH3AL), also known as Noc2, is a Rab effector protein with a molecular weight of 38 kDa. It is expressed abundantly in endocrine cells but at low levels in exocrine tissues, playing a direct regulatory role in calcium-ion-dependent exocytosis in both endocrine and exocrine cells, and also having a key role in INS secretion by pancreatic cells. RPH3AL functions as a potential RAB3B effector protein in epithelial cells, and RPH3AL gene is likely a tumor suppressor.