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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPL10 Polyclonal antibody, PBS Only

RPL10 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17013-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPL10 Polyclonal antibody (17013-1-AP)

    WB analysis using 17013-1-AP (same clone as 17013-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17013-1-AP (RPL10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17013-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RPL10 Polyclonal antibody (17013-1-AP)

    WB analysis using 17013-1-AP (same clone as 17013-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17013-1-AP (RPL10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17013-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RPL10 Polyclonal antibody (17013-1-AP)

    IHC staining of mouse brain using 17013-1-AP (same clone as 17013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17013-1-AP (RPL10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17013-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RPL10 Polyclonal antibody (17013-1-AP)

    IHC staining of mouse brain using 17013-1-AP (same clone as 17013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17013-1-AP (RPL10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17013-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using RPL10 Polyclonal antibody (17013-1-AP)

    IHC staining of human liver using 17013-1-AP (same clone as 17013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17013-1-AP (RPL10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17013-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using RPL10 Polyclonal antibody (17013-1-AP)

    IHC staining of human liver using 17013-1-AP (same clone as 17013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17013-1-AP (RPL10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17013-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17013-1-PBS targets RPL10 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL10 fusion protein Ag9969 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L10
Calculated molecular weight 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC003358
Gene symbol RPL10
Gene ID (NCBI) 6134
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RPL10, also named as Large ribosomal subunit protein uL16, is a 214 amino acid protein, which belongs to the universal ribosomal protein uL16 family. RPL10 is a component of the large ribosomal subunit and is down-regulated during adipocyte, kidney, and heart differentiation. RPL10 shares 98% amino acid sequence with RPL10L, so RPL10 antibody is able to recognize RPL10 and RPL10L protein.