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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPL12 Monoclonal antibody

RPL12 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C9B3

Cat no : 68110-1-Ig

Synonyms

ribosomal protein L12, Large ribosomal subunit protein uL11, 60S ribosomal protein L12, 2C9B3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPL12 Monoclonal antibody (68110-1-Ig)

    WB analysis using 68110-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68110-1-Ig (RPL12 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using RPL12 Monoclonal antibody (68110-1-Ig)

    IHC staining of human endometrial cancer using 68110-1-Ig

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RPL12 Monoclonal antibody (68110-1-Ig)

    IHC staining of mouse brain using 68110-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using RPL12 Monoclonal antibody (68110-1-Ig)

    IHC staining of mouse stomach using 68110-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using RPL12 Monoclonal antibody (68110-1-Ig)

    IHC staining of human placenta using 68110-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using RPL12 Monoclonal antibody (68110-1-Ig)

    IHC staining of rat brain using 68110-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat stomach tissue using RPL12 Monoclonal antibody (68110-1-Ig)

    IHC staining of rat stomach using 68110-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inmouse brain tissue, human endometrial cancer tissue, human placenta tissue, mouse stomach tissue, rat brain tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:8000-1:32000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68110-1-Ig targets RPL12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RPL12 fusion protein Ag6580 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L12
Calculated molecular weight 18 kDa
Observed molecular weight21 kDa
GenBank accession numberBC050644
Gene symbol RPL12
Gene ID (NCBI) 6136
RRIDAB_2923639
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RPL12 antibody 68110-1-IgDownload protocol
IHC protocol for RPL12 antibody 68110-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols