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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPL12 Monoclonal antibody (68110-1-Ig)

    WB analysis using 68110-1-Ig (same clone as 68110-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68110-1-Ig (RPL12 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68110-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using RPL12 Monoclonal antibody (68110-1-Ig)

    IHC staining of rat brain using 68110-1-Ig (same clone as 68110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68110-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat stomach tissue using RPL12 Monoclonal antibody (68110-1-Ig)

    IHC staining of rat stomach using 68110-1-Ig (same clone as 68110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68110-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using RPL12 Monoclonal antibody (68110-1-Ig)

    IHC staining of human placenta using 68110-1-Ig (same clone as 68110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68110-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using RPL12 Monoclonal antibody (68110-1-Ig)

    IHC staining of human endometrial cancer using 68110-1-Ig (same clone as 68110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68110-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RPL12 Monoclonal antibody (68110-1-Ig)

    IHC staining of mouse brain using 68110-1-Ig (same clone as 68110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68110-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using RPL12 Monoclonal antibody (68110-1-Ig)

    IHC staining of mouse stomach using 68110-1-Ig (same clone as 68110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 68110-1-Ig (RPL12 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68110-1-PBS in a different storage buffer formulation.

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RPL12 Monoclonal antibody, PBS Only

RPL12 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 68110-1-PBS
Clone No.2C9B3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

ribosomal protein L12, Large ribosomal subunit protein uL11, 60S ribosomal protein L12, 2C9B3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68110-1-PBS targets RPL12 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RPL12 fusion protein Ag6580 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L12
Calculated molecular weight 18 kDa
Observed molecular weight21 kDa
GenBank accession numberBC050644
Gene Symbol RPL12
Gene ID (NCBI) 6136
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP30050
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.