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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPL14 Recombinant antibody (82688-1-RR)

    WB analysis using 82688-1-RR (same clone as 82688-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82688-1-RR (RPL14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82688-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPL14 Recombinant antibody (82688-1-RR)

    IF Staining of HepG2 using 82688-1-RR (same clone as 82688-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPL14 antibody (82688-1-RR, Clone: 2K22 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82688-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82688-1-RR

    Affinity and Kinetic Characterization of 82688-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82688-1-RR against Human RPL14 were performed. The affinity constant is below 1 pM.

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RPL14 Recombinant antibody, PBS Only

RPL14 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 82688-1-PBS
Clone No.2K22

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

2K22, CAG ISL 7, CAG-ISL 7, CTG B33, hRL14

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82688-1-PBS targets RPL14 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RPL14 fusion protein Ag6976 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L14
Calculated molecular weight 23 kDa
Observed molecular weight23-27 kDa
GenBank accession numberBC000606
Gene Symbol RPL14
Gene ID (NCBI) 9045
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50914
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RPL14 is a component of the large ribosomal subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain.