Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, MCF-7 cells, A431 cells, MDA-MB-453s cells, Jurkat cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82688-1-RR targets RPL14 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | RPL14 fusion protein Ag6976 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal protein L14 |
| Calculated molecular weight | 23 kDa |
| Observed molecular weight | 23-27 kDa |
| GenBank accession number | BC000606 |
| Gene Symbol | RPL14 |
| Gene ID (NCBI) | 9045 |
| RRID | AB_3086505 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P50914 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RPL14 is a component of the large ribosomal subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RPL14 antibody 82688-1-RR | Download protocol |
| IF protocol for RPL14 antibody 82688-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |