CoraLite®594-conjugated RPL3 Monoclonal antibody
RPL3 Monoclonal Antibody for WB, IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
2G1B8
Cat no : CL594-66130
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-66130 targets RPL3 in WB, IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | RPL3 fusion protein Ag20400 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal protein L3 |
| Calculated molecular weight | 46/27 kDa |
| Observed molecular weight | 46 kDa |
| GenBank accession number | BC012786 |
| Gene symbol | RPL3 |
| Gene ID (NCBI) | 6122 |
| RRID | AB_2919951 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL3, also named as TARBP-B and MGC104284, belongs to the ribosomal protein L3P family. This protein is a component of the 60S subunit. It can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CL594 RPL3 antibody CL594-66130 | Download protocol |
| IF protocol for CL594 RPL3 antibody CL594-66130 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
