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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated RPL6 Monoclonal antibody

RPL6 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3F6F8

Cat no : CL594-67729

Synonyms

60S ribosomal protein L6, Neoplasm related protein C140, ribosomal protein L6, RPL6, SHUJUN 2, TAXREB107, TXREB1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated RPL6 Monoclonal antibody (CL594-67729)

    IF Staining of HepG2 using CL594-67729

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 RPL6 antibody (CL594-67729, Clone: 3F6F8 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67729 targets RPL6 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RPL6 fusion protein Ag6840 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L6
Calculated molecular weight 33 kDa
Observed molecular weight34 kDa
GenBank accession numberBC004138
Gene symbol RPL6
Gene ID (NCBI) 6128
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribosomes are large multiple protein complexes which is composed of more than 80 components and provide a protein-synthesizing machinery for all living cells. Ribosomal protein like 6 (RPL6, also called Taxreb107 for Tax responsive element binding protein 107), one of the ribosomal components, can bind with the Tax responsive element of human T cell leukemia virus (HTLV)-I in addition to be assembled to ribosomes [PMID:12031506]. Overexpressed in gastric cancer could promote cell growth and cell cycle progression at least through upregulating cyclin E expression[PMID:20171175].

Protocols

Product Specific Protocols
IF protocol for CL594 RPL6 antibody CL594-67729Download protocol
Standard Protocols
Click here to view our Standard Protocols