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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPL9 Monoclonal antibody (68054-1-Ig)

    WB analysis using 68054-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68054-1-Ig (RPL9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using RPL9 Monoclonal antibody (68054-1-Ig)

    IHC staining of mouse cerebellum using 68054-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68054-1-Ig (RPL9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using RPL9 Monoclonal antibody (68054-1-Ig)

    IHC staining of mouse cerebellum using 68054-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68054-1-Ig (RPL9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using RPL9 Monoclonal antibody (68054-1-Ig)

    IF Staining of mouse cerebellum using 68054-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using RPL9 antibody (68054-1-Ig, Clone: 2A6B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using RPL9 Monoclonal antibody (68054-1-Ig)

    IF Staining of mouse cerebellum using 68054-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using RPL9 antibody (68054-1-Ig, Clone: 2A6B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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RPL9 Monoclonal antibody

RPL9 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 68054-1-Ig
Clone No.2A6B11
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

2A6B11, 60S ribosomal protein L9, FLJ27456, Large ribosomal subunit protein uL6, OK/SW-cl.103

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, A549 cells, HepG2 cells, MCF-7 cells, U2OS cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

68054-1-Ig targets RPL9 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RPL9 fusion protein Ag5391 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L9
Calculated molecular weight 22 kDa
Observed molecular weight22 kDa
GenBank accession numberBC066318
Gene Symbol RPL9
Gene ID (NCBI) 6133
RRIDAB_2918795
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP32969
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RPL9 antibody 68054-1-IgDownload protocol
IHC protocol for RPL9 antibody 68054-1-IgDownload protocol
IF protocol for RPL9 antibody 68054-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

J Cardiovasc Transl Res

CREB1 Silencing Protects Against Inflammatory Response in Rats with Deep Vein Thrombosis Through Reducing RPL9 Expression and Blocking NF-κB Signaling

Authors - Xiaorong Jian
View Article