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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPL9 Monoclonal antibody, PBS Only

RPL9 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A6B11

Cat no : 68054-1-PBS

Synonyms

60S ribosomal protein L9, DKFZp313J1510, FLJ27456, ribosomal protein L9, RPL9, RPL9P7, RPL9P8, RPL9P9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPL9 Monoclonal antibody (68054-1-Ig)

    WB analysis using 68054-1-Ig (same clone as 68054-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68054-1-Ig (RPL9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68054-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using RPL9 Monoclonal antibody (68054-1-Ig)

    IHC staining of mouse cerebellum using 68054-1-Ig (same clone as 68054-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68054-1-Ig (RPL9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68054-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using RPL9 Monoclonal antibody (68054-1-Ig)

    IHC staining of mouse cerebellum using 68054-1-Ig (same clone as 68054-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68054-1-Ig (RPL9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68054-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using RPL9 Monoclonal antibody (68054-1-Ig)

    IF Staining of mouse cerebellum using 68054-1-Ig (same clone as 68054-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using RPL9 antibody (68054-1-Ig, Clone: 2A6B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68054-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using RPL9 Monoclonal antibody (68054-1-Ig)

    IF Staining of mouse cerebellum using 68054-1-Ig (same clone as 68054-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using RPL9 antibody (68054-1-Ig, Clone: 2A6B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68054-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68054-1-PBS targets RPL9 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RPL9 fusion protein Ag5391 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L9
Calculated molecular weight 22 kDa
Observed molecular weight22 kDa
GenBank accession numberBC066318
Gene symbol RPL9
Gene ID (NCBI) 6133
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.