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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPP30 Recombinant antibody (83481-5-RR)

    WB analysis using 83481-5-RR (same clone as 83481-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83481-5-RR (RPP30 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83481-5-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00472-1

    Sandwich ELISA standard curve of MP00472-1

    Sandwich ELISA standard curve of MP00472-1, Human RPP30 Recombinant Matched Antibody Pair - PBS only. 83481-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag8600. 83481-5-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 ng/mL

  • Sample test of MP00472-1

    Sample test of MP00472-1

    HEK-293 and U-251 cell lysate were measured. The human RPP30 concentration of detected samples was determined to be 194.20 ng/mL (based on a 1.2 mg/mL extract load) in HEK-293 cell lysate and 139.41 ng/mL (based on a 3.3 mg/mL extract load) in U-251 cell lysate.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using RPP30 Recombinant antibody (83481-5-RR)

    IHC staining of human ovary cancer using 83481-5-RR (same clone as 83481-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83481-5-RR (RPP30 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83481-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using RPP30 Recombinant antibody (83481-5-RR)

    IHC staining of human ovary cancer using 83481-5-RR (same clone as 83481-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83481-5-RR (RPP30 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83481-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using RPP30 Recombinant antibody (83481-5-RR)

    IF Staining of U-251 using 83481-5-RR (same clone as 83481-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using RPP30 antibody (83481-5-RR, Clone: 240468E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83481-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using RPP30 Recombinant antibody (83481-5-RR)

    IF Staining of U-251 using 83481-5-RR (same clone as 83481-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using RPP30 antibody (83481-5-RR, Clone: 240468E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83481-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-251 cells using RPP30 Recombinant antibody (83481-5-RR)

    FC experiment of U-251 using 83481-5-RR (same clone as 83481-5-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug RPP30 Recombinant antibody (83481-5-RR, Clone:240468E4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83481-5-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00472-1

    Cytometric bead array standard curve of MP00472-1

    Cytometric bead array standard curve of MP00472-1, RPP30 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83481-2-PBS. Detection antibody: 83481-5-PBS. Standard: Ag8600. Range: 0.78-100 ng/mL.

  • Affinity and Kinetic Characterization of 83481-5-RR

    Affinity and Kinetic Characterization of 83481-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83481-5-RR against Human RPP30 were performed. The affinity constant is below 1 pM.

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View 3D epitope

RPP30 Recombinant antibody, PBS Only (Detector)

RPP30 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83481-5-PBS
Clone No.240468E4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

TSG15, RNASEP2, RNaseP protein p30, RNase P subunit 2, 240468E4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1 mg/ml
100 μg, 1 mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83481-5-PBS targets RPP30 as part of a matched antibody pair:

MP00472-1: 83481-2-PBS capture and 83481-5-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RPP30 fusion protein Ag8600 相同性解析による交差性が予測される生物種
Full Name ribonuclease P/MRP 30kDa subunit
Calculated molecular weight 268 aa, 29 kDa
Observed molecular weight30 kDa
GenBank accession numberBC006991
Gene Symbol RPP30
Gene ID (NCBI) 10556
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP78346
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RPP30 is a part of multimeric ribonuclease P complex and ribonuclease MRP complex. It is also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences.