Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HEK-293 cells, NIH/3T3 cells, U-251 cells |
Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | U-251 cells |
Positive FC (Intra) detected in | U-251 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:750-1:3000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
83481-5-RR targets RPP30 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | RPP30 fusion protein Ag8600 相同性解析による交差性が予測される生物種 |
Full Name | ribonuclease P/MRP 30kDa subunit |
Calculated molecular weight | 268 aa, 29 kDa |
Observed molecular weight | 30 kDa |
GenBank accession number | BC006991 |
Gene Symbol | RPP30 |
Gene ID (NCBI) | 10556 |
RRID | AB_3671109 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P78346 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RPP30 is a part of multimeric ribonuclease P complex and ribonuclease MRP complex. It is also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences.
Protocols
Product Specific Protocols | |
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WB protocol for RPP30 antibody 83481-5-RR | Download protocol |
IHC protocol for RPP30 antibody 83481-5-RR | Download protocol |
IF protocol for RPP30 antibody 83481-5-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |