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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPS20 Monoclonal antibody (68270-1-Ig)

    WB analysis using 68270-1-Ig (same clone as 68270-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68270-1-Ig (RPS20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68270-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RPS20 Monoclonal antibody (68270-1-Ig)

    WB analysis using 68270-1-Ig (same clone as 68270-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68270-1-Ig (RPS20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68270-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RPS20 Monoclonal antibody (68270-1-Ig)

    IHC staining of human breast cancer using 68270-1-Ig (same clone as 68270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68270-1-Ig (RPS20 antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 68270-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RPS20 Monoclonal antibody (68270-1-Ig)

    IF Staining of HeLa using 68270-1-Ig (same clone as 68270-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RPS20 antibody (68270-1-Ig, Clone: 3E9B4 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68270-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50774-1

    Cytometric bead array standard curve of MP50774-1

    Cytometric bead array standard curve of MP50774-1, RPS20 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68270-2-PBS. Detection antibody: 68270-1-PBS. Standard:Ag8275. Range: 1.563-200 ng/mL.

  • Cytometric bead array standard curve of MP50774-2

    Cytometric bead array standard curve of MP50774-2

    Cytometric bead array standard curve of MP50774-2, RPS20 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68270-3-PBS. Detection antibody: 68270-1-PBS. Standard:Ag8275. Range: 3.125-200 ng/mL.

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RPS20 Monoclonal antibody, PBS Only (Detector)

RPS20 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 68270-1-PBS
Clone No.3E9B4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

Small ribosomal subunit protein uS10, ribosomal protein S20, 40S ribosomal protein S20, 3E9B4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68270-1-PBS targets RPS20 as part of a matched antibody pair:

MP50774-1: 68270-2-PBS capture and 68270-1-PBS detection (validated in Cytometric bead array)

MP50774-2: 68270-3-PBS capture and 68270-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RPS20 fusion protein Ag8275 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S20
Calculated molecular weight 13 kDa
Observed molecular weight16 kDa
GenBank accession numberBC007507
Gene Symbol RPS20
Gene ID (NCBI) 6224
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP60866
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS20 is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm.