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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPS20 Monoclonal antibody

RPS20 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3E9B4

Cat no : 68270-1-Ig

Synonyms

40S ribosomal protein S20, FLJ27451, ribosomal protein S20, RPS20

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPS20 Monoclonal antibody (68270-1-Ig)

    WB analysis using 68270-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68270-1-Ig (RPS20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using RPS20 Monoclonal antibody (68270-1-Ig)

    WB analysis using 68270-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68270-1-Ig (RPS20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RPS20 Monoclonal antibody (68270-1-Ig)

    IHC staining of human breast cancer using 68270-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68270-1-Ig (RPS20 antibody) at dilution of 1:1000 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RPS20 Monoclonal antibody (68270-1-Ig)

    IF Staining of HeLa using 68270-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RPS20 antibody (68270-1-Ig, Clone: 3E9B4 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inJurkat cells, HeLa cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, HEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:750-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68270-1-Ig targets RPS20 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RPS20 fusion protein Ag8275 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S20
Calculated molecular weight 13 kDa
Observed molecular weight16 kDa
GenBank accession numberBC007507
Gene symbol RPS20
Gene ID (NCBI) 6224
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS20 is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm.

Protocols

Product Specific Protocols
WB protocol for RPS20 antibody 68270-1-IgDownload protocol
IHC protocol for RPS20 antibody 68270-1-IgDownload protocol
IF protocol for RPS20 antibody 68270-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols