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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated RPS20 Monoclonal ant (CL488-68270)

    IF Staining of HeLa using CL488-68270

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 RPS20 antibody (CL488-68270, Clone: 3E9B4 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated RPS20 Monoclonal ant (CL488-68270)

    FC experiment of HeLa using CL488-68270

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RPS20 (CL488-68270, Clone:3E9B4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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CoraLite® Plus 488-conjugated RPS20 Monoclonal antibody

RPS20 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68270
Clone No.3E9B4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

Small ribosomal subunit protein uS10, ribosomal protein S20, 40S ribosomal protein S20, 3E9B4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68270 targets RPS20 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RPS20 fusion protein Ag8275 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S20
Calculated molecular weight 13 kDa
Observed molecular weight16 kDa
GenBank accession numberBC007507
Gene Symbol RPS20
Gene ID (NCBI) 6224
RRIDAB_3084463
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP60866
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS20 is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RPS20 antibody CL488-68270Download protocol
FC protocol for CL Plus 488 RPS20 antibody CL488-68270Download protocol
Standard Protocols
Click here to view our Standard Protocols