Validation Data Gallery
Filter:
Tested Applications
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-14946 targets RPS27A in IF/ICC applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RPS27A fusion protein Ag6750 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal protein S27a |
| Calculated molecular weight | 18 kDa |
| Observed molecular weight | 9 kDa, 18 kDa |
| GenBank accession number | BC001392 |
| Gene Symbol | RPS27A |
| Gene ID (NCBI) | 6233 |
| RRID | AB_3672612 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen Affinity Purified |
| UNIPROT ID | P62979 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RPS27A, also named as UBA80 and UBCEP1, can be cleaved into two chains Ubiquitin and 40S ribosomal protein S27a. Ubiquitin is a highly conserved 76-amino acid protein that plays a key role in protein degradation. Ubiquitin is synthesized as precursor proteins that consist either of polyubiquitin chains that are cleaved into moieties of the UBB or UBC types, or single ubiquitin moieties fused 5-prime to unrelated carboxyl extension proteins (UBA type) such as UBA52 and UBA80 (RPS27A) [PMID:10772958]
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 RPS27A antibody CL488-14946 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |