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KD/KO Validated

RPS3 Monoclonal antibody

Name: RPS3 Monoclonal antibody
Brand: Proteintech
SKU: 66046-1-Ig

RPS3 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

CloneNo.

2G7H4

Review Product

Cat no : 66046-1-Ig

Synonyms

40S ribosomal protein S3, Cell and organelle markers, ribosomal protein S3, Ribosome Marker, RPS3

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 66046-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.
WB analysis using 66046-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 66046-1-Ig
human brain tissue were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Neuro-2a using 66046-1-Ig
Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of RAW 264.7 using 66046-1-Ig
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of ROS1728 using 66046-1-Ig
ROS1728 cells were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 66046-1-Ig
rat brain tissue were subjected to SDS PAGE followed by western blot with 66046-1-Ig (RPS3 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 66046-1-Ig
IP result of anti-RPS3 (IP:66046-1-Ig, 3ug; Detection:66046-1-Ig 1:2000) with HEK-293 cells lysate 1800ug.
IHC staining of human ovary tumor using 66046-1-Ig
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66046-1-Ig (RPS3 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 66046-1-Ig
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66046-1-Ig (RPS3 Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 66046-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer using 66046-1-Ig(RPS3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 66046-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer using 66046-1-Ig(RPS3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human pancreas using 66046-1-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas using 66046-1-Ig(RPS3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 66046-1-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas using 66046-1-Ig(RPS3 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 66046-1-Ig
Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66046-1-Ig(RPS3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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Cat No. 66046-1-Ig

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HSC-T6 cells, HeLa cells, human brain tissue, Neuro-2a cells, RAW 264.7 cells, ROS1728 cells, rat brain tissue, HEK-293 cells, HepG2 cells, K-562 cells, Jurkat cells, NIH/3T3 cells, 4T1 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human ovary tumor tissue, human colon cancer tissue, human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:20000-1:100000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 10 publications below
IHC	See 3 publications below
IF	See 4 publications below
FC	See 1 publications below
ChIP	See 1 publications below

Product Information

66046-1-Ig targets RPS3 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	RPS3 fusion protein Ag7515 相同性解析による交差性が予測される生物種
Full Name	ribosomal protein S3
Calculated molecular weight	27 kDa
Observed molecular weight	33 kDa
GenBank accession number	BC003137
Gene symbol	RPS3
Gene ID (NCBI)	6188
RRID	AB_11182493
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival. The calculated molecular weight of RPS3 is 26 kDa, but the modified RPS3 is about 33 kDa.

Protocols

Product Specific Protocols
WB protocol for RPS3 antibody 66046-1-Ig	Download protocol
IHC protocol for RPS3 antibody 66046-1-Ig	Download protocol
IF protocol for RPS3 antibody 66046-1-Ig	Download protocol
IP protocol for RPS3 antibody 66046-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	FC	Transl Res Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication. Authors - Mahmoud Dabbah View Article
human	IHC	Proc Natl Acad Sci U S A Local translation in nuclear condensate amyloid bodies. Authors - Phaedra R Theodoridis View Article
human	WB	PLoS Pathog Separate domains of G3BP promote efficient clustering of alphavirus replication complexes and recruitment of the translation initiation machinery. Authors - Benjamin Götte View Article
human	WB	Free Radic Biol Med Identification of interacting partners of Human Mpv17-like protein with a mitigating effect of mitochondrial dysfunction through mtDNA damage. Authors - Reiko Iida View Article
human	WB	Oncotarget Ribosomal protein S3 (rpS3) secreted from various cancer cells is N-linked glycosylated. Authors - Yong Joong Kim KD Validated View Article
human	WB,IF	Front Immunol Interactome of the Autoimmune Risk Protein ANKRD55. Authors - Nerea Ugidos View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPS3 Monoclonal antibody

RPS3 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 66046-1-Ig

WB analysis using 66046-1-Ig

WB analysis of human brain using 66046-1-Ig

WB analysis of Neuro-2a using 66046-1-Ig

WB analysis of RAW 264.7 using 66046-1-Ig

WB analysis of ROS1728 using 66046-1-Ig

WB analysis of rat brain using 66046-1-Ig

IP experiment of HEK-293 using 66046-1-Ig

IHC staining of human ovary tumor using 66046-1-Ig

IHC staining of human ovary tumor using 66046-1-Ig

IHC staining of human colon cancer using 66046-1-Ig

IHC staining of human colon cancer using 66046-1-Ig

IHC staining of human pancreas using 66046-1-Ig

IHC staining of human pancreas using 66046-1-Ig

IF Staining of HepG2 using 66046-1-Ig

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.

Local translation in nuclear condensate amyloid bodies.

Separate domains of G3BP promote efficient clustering of alphavirus replication complexes and recruitment of the translation initiation machinery.

Identification of interacting partners of Human Mpv17-like protein with a mitigating effect of mitochondrial dysfunction through mtDNA damage.

Ribosomal protein S3 (rpS3) secreted from various cancer cells is N-linked glycosylated.

Interactome of the Autoimmune Risk Protein ANKRD55.