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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated RPS3 Monoclonal antibody

RPS3 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2G7H4

Cat no : CL488-66046

Synonyms

40S ribosomal protein S3, FLJ26283, FLJ27450, ribosomal protein S3, Ribosome Marker, RPS3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated RPS3 Monoclonal anti (CL488-66046)

    IF Staining of HepG2 using CL488-66046

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66046 (RPS3 antibody) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-66046 is RPS3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RPS3 fusion protein Ag7515 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S3
Calculated molecular weight 27 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC003137
Gene symbol RPS3
Gene ID (NCBI) 6188
RRIDAB_2883239
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival. The calculated molecular weight of RPS3 is 26 kDa, but the modified RPS3 is about 33 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RPS3 antibody CL488-66046Download protocol
Standard Protocols
Click here to view our Standard Protocols