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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated S6 Ribosomal protein Monoc (CL594-66886)

    IF Staining of HepG2 using CL594-66886

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66886 (S6 Ribosomal protein antibody) at dilution of 1:100.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CoraLite®594-conjugated S6 Ribosomal protein Monoclonal antibody

S6 Ribosomal protein Monoclonal Antibody for IF/ICC
Cat No. CL594-66886
Clone No.1C3E10

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

IF/ICC

Synonyms

40S ribosomal protein S6, Cell and organelle markers, Phosphoprotein NP33, ribosomal protein S6, Ribosome Marker, RPS6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66886 targets S6 Ribosomal protein in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen S6 Ribosomal protein fusion protein Ag6599 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S6
Calculated molecular weight 29 kDa
Observed molecular weight 29-32 kDa
GenBank accession numberBC000524
Gene Symbol RPS6
Gene ID (NCBI) 6194
RRIDAB_2883632
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62753
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

Protocols

Product Specific Protocols
IF protocol for CL594 S6 Ribosomal protein antibody CL594-66886Download protocol
Standard Protocols
Click here to view our Standard Protocols