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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated RRM1 Monoclonal antibody

RRM1 Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5H6F3

Cat no : CL488-60073

Synonyms

RIR1, ribonucleotide reductase M1, Ribonucleoside-diphosphate reductase subunit M1, Ribonucleoside-diphosphate reductase large subunit, EC:1.17.4.1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CoraLite® Plus 488-conjugated RRM1 Monoclonal anti (CL488-60073)

    IF Staining of human breast cancer using CL488-60073

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 488 RRM1 antibody (CL488-60073, Clone: 5H6F3 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated RRM1 Monoclonal anti (CL488-60073)

    IF Staining of HepG2 using CL488-60073

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 RRM1 antibody (CL488-60073, Clone: 5H6F3 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated RRM1 Monoclonal anti (CL488-60073)

    FC experiment of HepG2 using CL488-60073

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RRM1 (CL488-60073, Clone:5H6F3) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inhuman breast cancer tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60073 targets RRM1 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RRM1 fusion protein Ag0789 相同性解析による交差性が予測される生物種
Full Name ribonucleotide reductase M1
Calculated molecular weight 90 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC006498
Gene symbol RRM1
Gene ID (NCBI) 6240
RRIDAB_2883095
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribonucleoside-diphosphate reductase functions as a heterodimer of a large and a small subunits in deoxyribonucleotide synthesis. RRM1 constitutes to the large subunit (R1) of ribonucleotide reductase, and it can either form heterodimer with small subunit RRM or RRM2B(PMID:16376858). RRM1 provides the precursors necessary for DNA synthesis. RRM1 can not be detected in quiescent cells, while its mRNA and protein are present throughout the cell cycle in cycling cells(PMID:8188248). Researches showed that RRM1 is involved in carcinogenesis, tumor progression, and the resistance of non-small-cell lung cancer (NSCLC) to treatment. Low level expression of RRM1 in NSCLC is associated with poor survival(PMID:17314339).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RRM1 antibody CL488-60073Download protocol
FC protocol for CL Plus 488 RRM1 antibody CL488-60073Download protocol
Standard Protocols
Click here to view our Standard Protocols