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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RUNX2 Recombinant antibody, PBS Only

RUNX2 Recombinant Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1G5

Cat no : 82636-2-PBS

Synonyms

AML3, CBF alpha 1, CBFA1, CCD, CCD1, Oncogene AML 3, OSF 2, OSF2, PEA2 alpha A, PEA2aA, PEBP2 alpha A, PEBP2A, PEBP2A1, PEBP2A2, PEBP2aA, PEBP2aA1, RUNX2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using RUNX2 Recombinant antibody (82636-2-RR)

    WB analysis using 82636-2-RR (same clone as 82636-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82636-2-RR (RUNX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82636-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of ROS1728 cells using RUNX2 Recombinant antibody (82636-2-RR)

    IP experiment of ROS1728 using 82636-2-RR (same clone as 82636-2-PBS)

    IP result of anti-RUNX2 (IP:82636-2-RR, 4ug; Detection:82636-2-RR 1:500) with ROS1728 cells lysate 2080 ug. This data was developed using the same antibody clone with 82636-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82636-2-PBS targets RUNX2 in WB, IP, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name runt-related transcription factor 2
Calculated molecular weight 57 kDa
Observed molecular weight57-60 kDa
GenBank accession numberNM_004348
Gene symbol RUNX2
Gene ID (NCBI) 860
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

RUNX2, also named as AML3, CBFA1, OSF2 and PEBP2A, is a Transcription factor which involved in osteoblastic differentiation and skeletal morphogenesis. It is essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. RUNX2 inhibits MYST4-dependent transcriptional activation.