RUNX2 Recombinant antibody
RUNX2 Recombinant Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse and More (1)
Applications
WB, IP, ELISA
Conjugate
Unconjugated
CloneNo.
1G5
Cat no : 82636-2-RR
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | PC-3 cells, Saos-2 cells, C2C12 cells |
| Positive IP detected in | ROS1728 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
Product Information
82636-2-RR targets RUNX2 in WB, IP, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | runt-related transcription factor 2 |
| Calculated molecular weight | 57 kDa |
| Observed molecular weight | 57-60 kDa |
| GenBank accession number | NM_004348 |
| Gene symbol | RUNX2 |
| Gene ID (NCBI) | 860 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RUNX2, also named as AML3, CBFA1, OSF2 and PEBP2A, is a Transcription factor which involved in osteoblastic differentiation and skeletal morphogenesis. It is essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. RUNX2 inhibits MYST4-dependent transcriptional activation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RUNX2 antibody 82636-2-RR | Download protocol |
| IP protocol for RUNX2 antibody 82636-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Intractable Rare Dis Res Role of IFITM2 in osteogenic differentiation of C3H10T1/2 mesenchymal stem cells | ||
Bone CTSD upregulation as a key driver of spinal ligament abnormalities in spinal stenosis | ||
Arch Oral Biol Diagnostic significance of LncRNA MIAT in periodontitis and the molecular mechanisms influencing periodontal ligament fibroblasts via the miR-204-5p/DKK1 axis | ||
Life Sci Circ_0114581 promotes osteogenic differentiation of BMSCs via the MiR-155-5p/HNRNPA3 axis |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Charlotte (Verified Customer) (07-22-2024) | I used this antibody in WB in cells that differentiate into osteoblast lineage upon stimulation. I had a strong visible band at the expected size
 |
FH Udesh (Verified Customer) (11-26-2023) | Worked well for ADSCs at 1:1000
|
