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  • Featured Product
  • KD/KO Validated

RUVBL1 Polyclonal antibody, PBS Only

RUVBL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10210-2-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RUVBL1 Polyclonal antibody (10210-2-AP)

    WB analysis using 10210-2-AP (same clone as 10210-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10210-2-AP (RUVBL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10210-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using RUVBL1 Polyclonal antibody (10210-2-AP)

    IP experiment of HeLa using 10210-2-AP (same clone as 10210-2-PBS)

    IP result of anti-RUVBL1 (IP:10210-2-AP, 3ug; Detection:10210-2-AP 1:1000) with HeLa cells lysate 800ug. This data was developed using the same antibody clone with 10210-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using RUVBL1 Polyclonal antibody (10210-2-AP)

    IHC staining of mouse liver using 10210-2-AP (same clone as 10210-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10210-2-AP (RUVBL1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10210-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using RUVBL1 Polyclonal antibody (10210-2-AP)

    IHC staining of human ovary tumor using 10210-2-AP (same clone as 10210-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10210-2-AP (RUVBL1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10210-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using RUVBL1 Polyclonal antibody (10210-2-AP)

    IHC staining of human ovary tumor using 10210-2-AP (same clone as 10210-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10210-2-AP (RUVBL1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10210-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using RUVBL1 Polyclonal antibody (10210-2-AP)

    IHC staining of mouse liver using 10210-2-AP (same clone as 10210-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10210-2-AP (RUVBL1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10210-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using RUVBL1 Polyclonal antibody (10210-2-AP)

    IF Staining of A431 using 10210-2-AP (same clone as 10210-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RUVBL1 antibody (10210-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10210-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10210-2-PBS targets RUVBL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RUVBL1 fusion protein Ag0281 相同性解析による交差性が予測される生物種
Full Name RuvB-like 1 (E. coli)
Calculated molecular weight 456 aa, 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC002993
Gene symbol RUVBL1
Gene ID (NCBI) 8607
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RUVBL1 is a member of the AAA (ATPase associated with diverse cellular activities) family of proteins that is associated with several chromatin-remodelling complexes and has important roles in transcriptional regulation, the DNA damage response, telomerase activity, snoRNP assembly, cellular transformation and cancer metastasis[PMID: 21617703]. It consists of 456 amino acids (50 kDa) and interacts with TATA-binding protein (TBP), which is a central component for transcriptional regulation by forming complexes with various transcription regulators. RUVBL1 is a component of a large nuclear protein complex, possibly the RNA polymerase II holoenzyme. RUVBL1 is also highly homologous to RuvB proteins that function as a DNA helicase promote branch migration of the Holliday junction[PMID: 9588198]. This antibody is a rabbit polyclonal antibody to human RUVBL1.