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  • Featured Product
  • KD/KO Validated

RUVBL1 Polyclonal antibody, PBS Only

RUVBL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10210-2-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RUVBL1 Polyclonal antibody (10210-2-AP)

    WB analysis using 10210-2-AP (same clone as 10210-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10210-2-AP (RUVBL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10210-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using RUVBL1 Polyclonal antibody (10210-2-AP)

    IP experiment of HeLa using 10210-2-AP (same clone as 10210-2-PBS)

    IP result of anti-RUVBL1 (IP:10210-2-AP, 3ug; Detection:10210-2-AP 1:1000) with HeLa cells lysate 800ug. This data was developed using the same antibody clone with 10210-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using RUVBL1 Polyclonal antibody (10210-2-AP)

    IHC staining of mouse liver using 10210-2-AP (same clone as 10210-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10210-2-AP (RUVBL1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10210-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using RUVBL1 Polyclonal antibody (10210-2-AP)

    IHC staining of human ovary tumor using 10210-2-AP (same clone as 10210-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10210-2-AP (RUVBL1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10210-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using RUVBL1 Polyclonal antibody (10210-2-AP)

    IHC staining of human ovary tumor using 10210-2-AP (same clone as 10210-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10210-2-AP (RUVBL1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10210-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using RUVBL1 Polyclonal antibody (10210-2-AP)

    IHC staining of mouse liver using 10210-2-AP (same clone as 10210-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10210-2-AP (RUVBL1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10210-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using RUVBL1 Polyclonal antibody (10210-2-AP)

    IF Staining of A431 using 10210-2-AP (same clone as 10210-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RUVBL1 antibody (10210-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10210-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10210-2-PBS targets RUVBL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RUVBL1 fusion protein Ag0281 相同性解析による交差性が予測される生物種
Full Name RuvB-like 1 (E. coli)
Calculated molecular weight 456 aa, 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC002993
Gene symbol RUVBL1
Gene ID (NCBI) 8607
RRIDAB_2184405
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

RUVBL1 is a member of the AAA (ATPase associated with diverse cellular activities) family of proteins that is associated with several chromatin-remodelling complexes and has important roles in transcriptional regulation, the DNA damage response, telomerase activity, snoRNP assembly, cellular transformation and cancer metastasis[PMID: 21617703]. It consists of 456 amino acids (50 kDa) and interacts with TATA-binding protein (TBP), which is a central component for transcriptional regulation by forming complexes with various transcription regulators. RUVBL1 is a component of a large nuclear protein complex, possibly the RNA polymerase II holoenzyme. RUVBL1 is also highly homologous to RuvB proteins that function as a DNA helicase promote branch migration of the Holliday junction[PMID: 9588198]. This antibody is a rabbit polyclonal antibody to human RUVBL1.