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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RUVBL1 Monoclonal antibody

RUVBL1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F2D9

Cat no : 67750-1-Ig

Synonyms

49 kDa TBP interacting protein, ECP 54, ECP54, INO80 complex subunit H, INO80H, Nuclear matrix protein 238, PONTIN, Pontin 52, Pontin52, RuvB like 1, RuvB like 1 (E. coli), RUVBL1, RVB1, TAP54 alpha, TIH1, TIP49, TIP49A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RUVBL1 Monoclonal antibody (67750-1-Ig)

    WB analysis using 67750-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67750-1-Ig (RUVBL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RUVBL1 Monoclonal antibody (67750-1-Ig)

    WB analysis using 67750-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67750-1-Ig (RUVBL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inU2OS cells, HeLa cells, HT-1080 cells, HCT 116 cells, K-562 cells, A549 cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67750-1-Ig targets RUVBL1 in WB, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RUVBL1 fusion protein Ag16688 相同性解析による交差性が予測される生物種
Full Name RuvB-like 1 (E. coli)
Calculated molecular weight 456 aa, 50 kDa
Observed molecular weight50 kDa
GenBank accession numberBC002993
Gene symbol RUVBL1
Gene ID (NCBI) 8607
RRIDAB_2918519
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RUVBL1 is a member of the AAA (ATPase associated with diverse cellular activities) family of proteins that is associated with several chromatin-remodelling complexes and has important roles in transcriptional regulation, the DNA damage response, telomerase activity, snoRNP assembly, cellular transformation and cancer metastasis[PMID: 21617703]. It consists of 456 amino acids (50 kDa) and interacts with TATA-binding protein (TBP), which is a central component for transcriptional regulation by forming complexes with various transcription regulators. RUVBL1 is a component of a large nuclear protein complex, possibly the RNA polymerase II holoenzyme. RUVBL1 is also highly homologous to RuvB proteins that function as a DNA helicase promote branch migration of the Holliday junction[PMID: 9588198].

Protocols

Product Specific Protocols
WB protocol for RUVBL1 antibody 67750-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols