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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated RUVBL1 Monoclonal antibody

RUVBL1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F2D9

Cat no : CL488-67750

Synonyms

ECP54, ECP 54, EC:3.6.4.12, 49 kDa TBP-interacting protein, 49 kDa TBP interacting protein

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of LNCaP cells using CoraLite® Plus 488-conjugated RUVBL1 Monoclonal an (CL488-67750)

    IF Staining of LNCaP using CL488-67750

    Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using CoraLite® Plus 488 RUVBL1 antibody (CL488-67750, Clone: 1F2D9 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite® Plus 488-conjugated RUVBL1 Monoclonal an (CL488-67750)

    FC experiment of HEK-293 using CL488-67750

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RUVBL1 (CL488-67750, Clone:1F2D9) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inLNCaP cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67750 targets RUVBL1 in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RUVBL1 fusion protein Ag16688 相同性解析による交差性が予測される生物種
Full Name RuvB-like 1 (E. coli)
Calculated molecular weight 456 aa, 50 kDa
Observed molecular weight50 kDa
GenBank accession numberBC002993
Gene symbol RUVBL1
Gene ID (NCBI) 8607
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RUVBL1 is a member of the AAA (ATPase associated with diverse cellular activities) family of proteins that is associated with several chromatin-remodelling complexes and has important roles in transcriptional regulation, the DNA damage response, telomerase activity, snoRNP assembly, cellular transformation and cancer metastasis[PMID: 21617703]. It consists of 456 amino acids (50 kDa) and interacts with TATA-binding protein (TBP), which is a central component for transcriptional regulation by forming complexes with various transcription regulators. RUVBL1 is a component of a large nuclear protein complex, possibly the RNA polymerase II holoenzyme. RUVBL1 is also highly homologous to RuvB proteins that function as a DNA helicase promote branch migration of the Holliday junction[PMID: 9588198].

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RUVBL1 antibody CL488-67750Download protocol
FC protocol for CL Plus 488 RUVBL1 antibody CL488-67750Download protocol
Standard Protocols
Click here to view our Standard Protocols