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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RXRB Recombinant antibody, PBS Only

RXRB Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242178D4

Cat no : 84656-4-PBS

Synonyms

Retinoic acid receptor RXR-beta, RCoR 1, NR2B2, H 2RIIBP, DAUDI6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RXRB Recombinant antibody (84656-4-RR)

    WB analysis using 84656-4-RR (same clone as 84656-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84656-4-RR (RXRB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84656-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using RXRB Recombinant antibody (84656-4-RR)

    IF Staining of MCF-7 using 84656-4-RR (same clone as 84656-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RXRB antibody (84656-4-RR, Clone: 242178D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84656-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using RXRB Recombinant antibody (84656-4-RR)

    FC experiment of HeLa using 84656-4-RR (same clone as 84656-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RXRB Recombinant antibody (84656-4-RR, Clone:242178D4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84656-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84656-4-RR

    Affinity and Kinetic Characterization of 84656-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84656-4-RR against Human RXRB were performed. The affinity constant is 1.24 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84656-4-PBS targets RXRB in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RXRB fusion protein Ag0263 相同性解析による交差性が予測される生物種
Full Name retinoid X receptor, beta
Calculated molecular weight 57 kDa
GenBank accession numberBC001167
Gene symbol RXRB
Gene ID (NCBI) 6257
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Retinoid X Receptor beta (RXRB) is a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the effects of retinoic acid. RXRB heterodimerizes with other nuclear hormone receptors, such as RAR, the thyroid hormone receptor, and the vitamin D3 receptor, to enhance binding to their cognate response elements. (PMID: 8257090)