• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RXRB Recombinant antibody

RXRB Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

242178D4

Cat no : 84656-4-RR

Synonyms

Retinoic acid receptor RXR-beta, RCoR 1, NR2B2, H 2RIIBP, DAUDI6

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using RXRB Recombinant antibody (84656-4-RR)

    WB analysis using 84656-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84656-4-RR (RXRB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using RXRB Recombinant antibody (84656-4-RR)

    IF Staining of MCF-7 using 84656-4-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RXRB antibody (84656-4-RR, Clone: 242178D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using RXRB Recombinant antibody (84656-4-RR)

    FC experiment of HeLa using 84656-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RXRB Recombinant antibody (84656-4-RR, Clone:242178D4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84656-4-RR

    Affinity and Kinetic Characterization of 84656-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84656-4-RR against Human RXRB were performed. The affinity constant is 1.24 nM.

☆ 2/28（金）まで！最大30%オフ！ >> トライアルサイズもお得な一次抗体キャンペーン

Tested Applications

Positive WB detected inSK-BR-3 cells, K-562 cells
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84656-4-RR targets RXRB in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RXRB fusion protein Ag0263 相同性解析による交差性が予測される生物種
Full Name retinoid X receptor, beta
Calculated molecular weight 57 kDa
GenBank accession numberBC001167
Gene symbol RXRB
Gene ID (NCBI) 6257
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Retinoid X Receptor beta (RXRB) is a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the effects of retinoic acid. RXRB heterodimerizes with other nuclear hormone receptors, such as RAR, the thyroid hormone receptor, and the vitamin D3 receptor, to enhance binding to their cognate response elements. (PMID: 8257090)

Protocols

Product Specific Protocols
WB protocol for RXRB antibody 84656-4-RRDownload protocol
IF protocol for RXRB antibody 84656-4-RRDownload protocol
FC protocol for RXRB antibody 84656-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols