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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RYBP Monoclonal antibody (68130-1-Ig)

    WB analysis using 68130-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68130-1-Ig (RYBP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of RAW 264.7 cells using RYBP Monoclonal antibody (68130-1-Ig)

    WB analysis of RAW 264.7 using 68130-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68130-1-Ig (RYBP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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RYBP Monoclonal antibody

RYBP Monoclonal Antibody for WB, ELISA
Cat No. 68130-1-Ig
Clone No.2C6G7

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, ELISA

Synonyms

AAP1, APAP 1, Apoptin associating protein 1, DED associated factor, DEDAF, RING1 and YY1 binding protein, RYBP, YEAF1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, RAW 264.7 cells, HeLa cells, U2OS cells, HEK-293 cells, LNCaP cells, Jurkat cells, Daudi cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68130-1-Ig targets RYBP in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RYBP fusion protein Ag31715 相同性解析による交差性が予測される生物種
Full Name RING1 and YY1 binding protein
Calculated molecular weight 228 aa, 25 kDa
Observed molecular weight32 kDa
GenBank accession numberBC014959
Gene Symbol RYBP
Gene ID (NCBI) 23429
RRIDAB_2923657
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8N488
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Polycomb repressive complexes (PRCs) are important chromatin regulators of embryonic stem (ES) cell function [PMID:22269950]. RYBP binds Polycomb H2A monoubiquitin ligases Ring1A and Ring1B and has been suggested to assist PRC localization to their targets. RYBP, firstly identified as a direct interactor with Ring1A, acts as transcriptional repressor in reporter assays, both in tissue culture cells and in the fly [PMID:16125914]

Protocols

Product Specific Protocols
WB protocol for RYBP antibody 68130-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols