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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RYBP Monoclonal antibody (68130-1-Ig)

    WB analysis using 68130-1-Ig (same clone as 68130-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68130-1-Ig (RYBP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68130-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using RYBP Monoclonal antibody (68130-1-Ig)

    WB analysis of RAW 264.7 using 68130-1-Ig (same clone as 68130-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68130-1-Ig (RYBP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68130-1-PBS in a different storage buffer formulation.

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RYBP Monoclonal antibody, PBS Only

RYBP Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68130-1-PBS
Clone No.2C6G7

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, Indirect ELISA

Synonyms

AAP1, APAP 1, Apoptin associating protein 1, DED associated factor, DEDAF, RING1 and YY1 binding protein, RYBP, YEAF1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68130-1-PBS targets RYBP in WB, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RYBP fusion protein Ag31715 相同性解析による交差性が予測される生物種
Full Name RING1 and YY1 binding protein
Calculated molecular weight 228 aa, 25 kDa
Observed molecular weight32 kDa
GenBank accession numberBC014959
Gene Symbol RYBP
Gene ID (NCBI) 23429
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8N488
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Polycomb repressive complexes (PRCs) are important chromatin regulators of embryonic stem (ES) cell function [PMID:22269950]. RYBP binds Polycomb H2A monoubiquitin ligases Ring1A and Ring1B and has been suggested to assist PRC localization to their targets. RYBP, firstly identified as a direct interactor with Ring1A, acts as transcriptional repressor in reporter assays, both in tissue culture cells and in the fly [PMID:16125914]