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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated RYR1 Monoclonal antibody

RYR1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B4E12

Cat no : CL488-66539

Synonyms

CCO, MHS, MHS1, Ryanodine receptor 1, RYDR, RYR, RYR 1, RYR1, SKRR

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using CoraLite® Plus 488-conjugated RYR1 Monoclonal anti (CL488-66539)

    IF Staining of PC-12 using CL488-66539

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using CoraLite® Plus 488 RYR1 antibody (CL488-66539, Clone: 1B4E12 ) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inPC-12 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66539 targets RYR1 in IF/ICC applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RYR1 fusion protein Ag25120 相同性解析による交差性が予測される生物種
Full Name ryanodine receptor 1 (skeletal)
Calculated molecular weight 565 kDa
GenBank accession numberNM_000540
Gene symbol RYR1
Gene ID (NCBI) 6261
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ryanodine receptor isoform-1 (RyR1) is a major calcium channel in skeletal muscle important for excitation-contraction coupling. The transmembrane region of RyR1 is comprised of two domains including the pseudo voltage sensor domain and the pore-forming domain (PMID: 27855725). Dominant RyR1 mutations are the leading cause of MH. Studies on the Ca2+ conducting properties of RyR1 channels containing Malignant hyperthermia mutations expressed in myotubes.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RYR1 antibody CL488-66539Download protocol
FC protocol for CL Plus 488 RYR1 antibody CL488-66539Download protocol
Standard Protocols
Click here to view our Standard Protocols