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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RYR2 Polyclonal antibody

RYR2 Polyclonal Antibody for IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 27587-1-AP

Synonyms

ARVC2, ARVD2, ryanodine receptor 2 (cardiac), RYR2, VTSIP

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human heart tissue using RYR2 Polyclonal antibody (27587-1-AP)

    IHC staining of human heart using 27587-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27587-1-AP (RYR2 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using RYR2 Polyclonal antibody (27587-1-AP)

    IHC staining of human heart using 27587-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27587-1-AP (RYR2 antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using RYR2 Polyclonal antibody (27587-1-AP)

    IF Staining of mouse heart using 27587-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using RYR2 antibody (27587-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using RYR2 Polyclonal antibody (27587-1-AP)

    IF Staining of mouse heart using 27587-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using RYR2 antibody (27587-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HEK-293T cells using RYR2 Polyclonal antibody (27587-1-AP)

    FC experiment of HEK-293T using 27587-1-AP

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human RYR2 (27587-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue
Positive FC (Intra) detected inHEK-293T cells
Positive FC detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27587-1-AP targets RYR2 in IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RYR2 fusion protein Ag26296 相同性解析による交差性が予測される生物種
Full Name ryanodine receptor 2 (cardiac)
Calculated molecular weight 565 kDa
GenBank accession numberNM_001035
Gene symbol RYR2
Gene ID (NCBI) 6262
RRIDAB_2880916
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RYR2 belongs to the ryanodine receptor family. RYR2 provides communication between transverse-tubules and sarcoplasmic reticulum. Contraction of cardiac muscle is triggered by release of calcium ions from SR following depolarization of T-tubules. Defects in RYR2 are the cause of familial arrhythmogenic right ventricular dysplasia type 2 (ARVD2) which known as arrhythmogenic right ventricular cardiomyopathy 2 (ARVC2). Defects in RYR2 are the cause of catecholaminergic polymorphic ventricular tachycardia type 1 (CPVT1) which known as stress-induced polymorphic ventricular tachycardia (VTSIP).

Protocols

Product Specific Protocols
IHC protocol for RYR2 antibody 27587-1-APDownload protocol
IF protocol for RYR2 antibody 27587-1-APDownload protocol
FC protocol for RYR2 antibody 27587-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols