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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Ribosomal protein L4 Recombinant antibody, PBS Only

Ribosomal protein L4 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3K6

Cat no : 80959-1-PBS

Synonyms

RPL4, RPL1, RPL 4, 60S ribosomal protein L4, 60S ribosomal protein L1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Ribosomal protein L4 Recombinant antibody (80959-1-RR)

    WB analysis using 80959-1-RR (same clone as 80959-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80959-1-RR (RPL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using Ribosomal protein L4 Recombinant antibody (80959-1-RR)

    IP experiment of HeLa using 80959-1-RR (same clone as 80959-1-PBS)

    IP result of anti-Ribosomal protein L4 (IP:80959-1-RR, 4ug; Detection:80959-1-RR 1:2000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Ribosomal protein L4 Recombinant antibody (80959-1-RR)

    IHC staining of human stomach cancer using 80959-1-RR (same clone as 80959-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 80959-1-RR (Ribosomal protein L4 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using Ribosomal protein L4 Recombinant antibody (80959-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 80959-1-RR (same clone as 80959-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 80959-1-RR (Ribosomal protein L4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Ribosomal protein L4 Recombinant antibody (80959-1-RR)

    IF Staining of HepG2 using 80959-1-RR (same clone as 80959-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Ribosomal protein L4 antibody (80959-1-RR, Clone: 3K6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Ribosomal protein L4 Recombinant antibody (80959-1-RR)

    IF Staining of HepG2 using 80959-1-RR (same clone as 80959-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Ribosomal protein L4 antibody (80959-1-RR, Clone: 3K6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using Ribosomal protein L4 Recombinant antibody (80959-1-RR)

    FC experiment of HepG2 using 80959-1-RR (same clone as 80959-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Ribosomal protein L4 (80959-1-RR, Clone:3K6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80959-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80959-1-PBS targets Ribosomal protein L4 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Ribosomal protein L4 fusion protein Ag28728 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L4
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC009888
Gene symbol Ribosomal protein L4
Gene ID (NCBI) 6124
RRIDAB_2918922
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.