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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Ribosomal protein L4 Recombinant antibody (80959-1-RR)

    WB analysis using 80959-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80959-1-RR (RPL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using Ribosomal protein L4 Recombinant antibody (80959-1-RR)

    IP experiment of HeLa using 80959-1-RR

    IP result of anti-Ribosomal protein L4 (IP:80959-1-RR, 4ug; Detection:80959-1-RR 1:2000) with HeLa cells lysate 1560 ug.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Ribosomal protein L4 Recombinant antibody (80959-1-RR)

    IHC staining of human stomach cancer using 80959-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 80959-1-RR (Ribosomal protein L4 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using Ribosomal protein L4 Recombinant antibody (80959-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 80959-1-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 80959-1-RR (Ribosomal protein L4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Ribosomal protein L4 Recombinant antibody (80959-1-RR)

    IF Staining of HepG2 using 80959-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Ribosomal protein L4 antibody (80959-1-RR, Clone: 3K6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Ribosomal protein L4 Recombinant antibody (80959-1-RR)

    IF Staining of HepG2 using 80959-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Ribosomal protein L4 antibody (80959-1-RR, Clone: 3K6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using Ribosomal protein L4 Recombinant antibody (80959-1-RR)

    FC experiment of HepG2 using 80959-1-RR

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Ribosomal protein L4 (80959-1-RR, Clone:3K6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Ribosomal protein L4 Recombinant antibody

Ribosomal protein L4 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 80959-1-RR
Clone No.3K6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

RPL4, RPL1, RPL 4, 60S ribosomal protein L4, 60S ribosomal protein L1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, U2OS cells, Jurkat cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80959-1-RR targets Ribosomal protein L4 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Ribosomal protein L4 fusion protein Ag28728 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L4
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC009888
Gene Symbol Ribosomal protein L4
Gene ID (NCBI) 6124
RRIDAB_2918922
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36578
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.

Protocols

Product Specific Protocols
WB protocol for Ribosomal protein L4 antibody 80959-1-RRDownload protocol
IHC protocol for Ribosomal protein L4 antibody 80959-1-RRDownload protocol
IF protocol for Ribosomal protein L4 antibody 80959-1-RRDownload protocol
IP protocol for Ribosomal protein L4 antibody 80959-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols