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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

S100B Recombinant antibody

S100B Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1E2

Cat no : 82271-8-RR

Synonyms

S100 beta, S-100 protein beta chain, S100 calcium-binding protein B, Protein S100-B, Protein S100 B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using S100 Beta Recombinant antibody (82271-8-RR)

    WB analysis using 82271-8-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82271-8-RR (S100B antibody) at dilution of 1:5150 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A375 cells using S100B Recombinant antibody (82271-8-RR)

    FC experiment of A375 using 82271-8-RR

    1x10^6 A375 cells were intracellularly stained with 0.25 ug S100B Recombinant antibody (82271-8-RR, Clone:1E2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82271-8-RR

    Affinity and Kinetic Characterization of 82271-8-RR

    Biolayer interferometry (BLl) kinetic assays of 82271-8-RR against Human S100 Beta were performed. The affinity constant is 38.5 pM.

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Tested Applications

Positive WB detected inC6 cells, A375 cells, rat brain tissue
Positive FC (Intra) detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10300
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82271-8-RR targets S100B in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen S100B fusion protein Ag7440 相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein B
Calculated molecular weight 11 kDa
Observed molecular weight11 kDa
GenBank accession numberBC001766
Gene symbol S100 Beta
Gene ID (NCBI) 6285
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs and has been implicated in the regulation of cellular activities such as metabolism, motility and proliferation. In the nervous system, S100B is constitutively released by astrocytes into the extracellular space and at nanomolar concentrations, it can promote neurite outgrowth and protect neurons against oxidative stress. Within the central nervous system (CNS), S100B is thought to be a marker for astroglial activation, linking astrocyte dysfunction to schizophrenia. In addition to astrocytes, S100B is released from many cell types, such as, adipocytes, chondrocytes, cardiomyocytes and lymphocytes. Serum S100B represents a new biomarker for mood disorders.

Protocols

Product Specific Protocols
WB protocol for S100B antibody 82271-8-RRDownload protocol
FC protocol for S100B antibody 82271-8-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols