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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using S100B Recombinant antibody (82271-8-RR)

    WB analysis using 82271-8-RR (same clone as 82271-8-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82271-8-RR (S100B antibody) at dilution of 1:5150 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82271-8-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of Human appendicitis tissue using S100B Recombinant antibody (82271-8-RR)

    IHC staining of Human appendicitis using 82271-8-RR (same clone as 82271-8-PBS)

    Immunohistochemical analysis of paraffin-embedded Human appendicitis tissue slide using 82271-8-RR (S100B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82271-8-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of Human appendicitis tissue using S100B Recombinant antibody (82271-8-RR)

    IHC staining of Human appendicitis using 82271-8-RR (same clone as 82271-8-PBS)

    Immunohistochemical analysis of paraffin-embedded Human appendicitis tissue slide using 82271-8-RR (S100B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82271-8-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A375 cells using S100B Recombinant antibody (82271-8-RR)

    FC experiment of A375 using 82271-8-RR (same clone as 82271-8-PBS)

    1x10^6 A375 cells were intracellularly stained with 0.25 ug S100B Recombinant antibody (82271-8-RR, Clone:1E2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82271-8-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82271-8-RR

    Affinity and Kinetic Characterization of 82271-8-RR

    Biolayer interferometry (BLl) kinetic assays of 82271-8-RR against Human S100 Beta were performed. The affinity constant is 38.5 pM.

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S100B Recombinant antibody, PBS Only

S100B Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 82271-8-PBS
Clone No.1E2

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

S100 beta, S-100 protein beta chain, S100 calcium-binding protein B, Protein S100-B, Protein S100 B

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82271-8-PBS targets S100B in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen S100B fusion protein Ag7440 相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein B
Calculated molecular weight 11 kDa
Observed molecular weight11 kDa
GenBank accession numberBC001766
Gene Symbol S100 Beta
Gene ID (NCBI) 6285
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04271
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs and has been implicated in the regulation of cellular activities such as metabolism, motility and proliferation. In the nervous system, S100B is constitutively released by astrocytes into the extracellular space and at nanomolar concentrations, it can promote neurite outgrowth and protect neurons against oxidative stress. Within the central nervous system (CNS), S100B is thought to be a marker for astroglial activation, linking astrocyte dysfunction to schizophrenia. In addition to astrocytes, S100B is released from many cell types, such as, adipocytes, chondrocytes, cardiomyocytes and lymphocytes. Serum S100B represents a new biomarker for mood disorders.