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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated S100A10 Monoclonal antibody

S100A10 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B3F9

Cat no : CL488-66227

Synonyms

42C, ANX2L, ANX2LG, Ca[1], CAL1L, Calpactin 1 light chain, Calpactin I light chain, Cellular ligand of annexin II, CLP11, GP11, p10, p10 protein, P11, Protein S100 A10, S100A10

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated S100A10 Monoclonal a (CL488-66227)

    IF Staining of A431 using CL488-66227

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®®488 S100A10 antibody (CL488-66227, Clone: 1B3F9 ) at dilution of 1:200, CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of Saos-2 cells using CoraLite® Plus 488-conjugated S100A10 Monoclonal a (CL488-66227)

    IF Staining of Saos-2 using CL488-66227

    Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using CoraLite® Plus 488 S100A10 antibody (CL488-66227, Clone: 1B3F9 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inA431 cells, Saos-2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66227 targets S100A10 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen S100A10 fusion protein Ag23596 相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A10
Calculated molecular weight 11 kDa
GenBank accession numberBC015973
Gene symbol S100A10
Gene ID (NCBI) 6281
RRIDAB_2919303
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S100A10, also known as p11, is a member of the S100 family of small, EF hand containing dimeric proteins. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A10 is present on the surface of endothelial and other cells in a heterotetrameric complex with another Ca(2+)-binding protein, annexin II. S100A10 may function in exocytosis and endocytosis.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 S100A10 antibody CL488-66227Download protocol
Standard Protocols
Click here to view our Standard Protocols