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S100A11 Polyclonal antibody

S100A11 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10237-1-AP

Synonyms

Calgizzarin, MLN 70, MLN70, Protein S100 A11, Protein S100 C, S100A11, S100C



Tested Applications

Positive WB detected inDU 145 cells, HCT 116 cells, BxPC-3 cells, human lung tissue, HEK-293 cells, human heart tissue, COLO 320 cells, HaCaT cells, U2OS cells, SKOV-3 cells
Positive IP detected inDU 145 cells, PC-3 cells
Positive IHC detected inhuman breast cancer tissue, human ovary tumor tissue, human colon cancer tissue, mouse liver tissue, human cervical cancer tissue, human lung cancer tissue, human thyroid cancer tissue, mouse kidney tissue, rat kidney tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells, BxPC-3 cells
Positive FC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy S100A11 Ready-To-Use IHC Kit. S100A11 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

10237-1-AP targets S100A11 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen S100A11 fusion protein Ag0343 相同性解析による交差性が予測される生物種
Full Name S100 calcium binding protein A11
Calculated molecular weight 105 aa, 12 kDa
Observed molecular weight 12 kDa
GenBank accession numberBC001410
Gene symbol S100A11
Gene ID (NCBI) 6282
RRIDAB_2183478
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

S100A11 (also known as S100C or calziggarin), is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs (PMID: 18694925). S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. First discovered in 1989, S100A11 has since been proposed to have direct biological functions in an assortment of physiological processes such as endo- and exocytosis, regulation of enzyme activity, cell growth and regulation, apoptosis and inflammation (PMID: 15241500). Chromosomal rearrangements and altered expression of S100A11 have been implicated in tumor metastasis. This S100A11 antibody (10237-1-AP) has also been instrumental in investigations into S100A11's role in the development of brain tumors in tuberous sclerosis complex (TSC) patients (PMID: 20133820).

Protocols

Product Specific Protocols
WB protocol for S100A11 antibody 10237-1-APDownload protocol
IHC protocol for S100A11 antibody 10237-1-APDownload protocol
IF protocol for S100A11 antibody 10237-1-APDownload protocol
IP protocol for S100A11 antibody 10237-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nat Commun

S100A11 is required for efficient plasma membrane repair and survival of invasive cancer cells.

Authors - Jyoti K Jaiswal
humanWB

Dev Cell

Annexin A1 Tethers Membrane Contact Sites that Mediate ER to Endosome Cholesterol Transport.

Authors - Emily R Eden
humanIHC

J Pathol

Tissue-based proteomics reveals FXYD3, S100A11 and GSTM3 as novel markers for regional lymph node metastasis in colon cancer.

Authors - Meding Stephan S
humanIF

Mol Cell Proteomics

S100A11 mediates hypoxia-induced mitogenic factor (HIMF)-induced smooth muscle cell migration, vesicular exocytosis, and nuclear activation.

Authors - Fan Chunling C
humanWB, IF, CoIP

Cell Mol Gastroenterol Hepatol

S100A11 Promotes Liver Steatosis via FOXO1-Mediated Autophagy and Lipogenesis.

Authors - Linqiang Zhang
human, mouseWB, IHC, IF

J Clin Endocrinol Metab

Down-regulation of S100A11, a calcium-binding protein, in human endometrium may cause reproductive failure.

Authors - Liu Xin-Mei XM