Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 10237-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10237-1-AP (S100A11 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 10237-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10237-1-AP (S100A11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 cells using 10237-1-AP
WB result of S100A11 antibody (10237-1-AP, 1:4000) with si-control and si-S100A11 transfected PC-3 cells.
WB analysis using 10237-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10237-1-AP (S100A11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 10237-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 10237-1-AP (S100A11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human lung using 10237-1-AP
human lung tissue were subjected to SDS PAGE followed by western blot with 10237-1-AP (S100A11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10237-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10237-1-AP (S100A11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 10237-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 10237-1-AP (S100A11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 10237-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 10237-1-AP (S100A11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of BxPC-3 using 10237-1-AP
BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10237-1-AP (S100A11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of PC-12 using 10237-1-AP
PC-12 cells were subjected to SDS PAGE followed by western blot with 10237-1-AP (S100A11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of PC-3 using 10237-1-AP
IP result of anti-S100A11 (IP:10237-1-AP, 3ug; Detection:10237-1-AP 1:1000) with PC-3 cells lysate 1000ug.
IP experiment of DU 145 using 10237-1-AP
IP result of anti-S100A11 (IP:10237-1-AP, 4ug; Detection:10237-1-AP 1:5000) with DU 145 cells lysate 1280 ug.
IHC staining of human cervical cancer using 10237-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10237-1-AP (S100A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 10237-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10237-1-AP (S100A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thyroid cancer using 10237-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10237-1-AP (S100A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 10237-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10237-1-AP (S100A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 10237-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10237-1-AP (S100A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 10237-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10237-1-AP (S100A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10237-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10237-1-AP (S100A11 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10237-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10237-1-AP (S100A11 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 10237-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10237-1-AP (S100A11 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 10237-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10237-1-AP (S100A11 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 10237-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10237-1-AP (S100A11 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10237-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 10237-1-AP (S100A11 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 10237-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 10237-1-AP (S100A11 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of PC-3 using 10237-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using S100A11 antibody (10237-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of BxPC-3 using 10237-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using S100A11 antibody (10237-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of BxPC-3 using 10237-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed BxPC-3 cells using S100A11 antibody (10237-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of PC-3 using 10237-1-AP
1x10^6 PC-3 cells were intracellularly stained with 0.8 ug S100A11 Polyclonal antibody (10237-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

S100A11 Polyclonal antibody

S100A11 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10237-1-AP

Publications(65)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, Neutralization, ELISA

Synonyms

Calgizzarin, MLN 70, MLN70, Protein S100 A11, Protein S100 C

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®555

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (65)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	DU 145 cells, HCT 116 cells, BxPC-3 cells, human lung tissue, HEK-293 cells, human heart tissue, COLO 320 cells, HaCaT cells, U2OS cells, SKOV-3 cells
Positive IP detected in	DU 145 cells, PC-3 cells
Positive IHC detected in	human breast cancer tissue, human cervical cancer tissue, human colon tissue, human colon cancer tissue, human liver cancer tissue, human lung cancer tissue, human ovary tumor tissue, human thyroid cancer tissue, mouse kidney tissue, rat kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	PC-3 cells, BxPC-3 cells
Positive FC (Intra) detected in	PC-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 8 publications below
WB	See 45 publications below
IHC	See 20 publications below
IF	See 19 publications below
IP	See 2 publications below
CoIP	See 2 publications below

Product Information

10237-1-AP targets S100A11 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, Neutralization, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0343 Product name: Recombinant human S100A11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 4-105 aa of BC001410 Sequence: ISSPTETERCIESLIAVFQKYAGKDGYNYTLSKTEFLSFMNTELAAFTKNQKDPGVLDRMMKKLDTNSDGQLDFSEFLNLIGGLAMACHDSFLKAVPSQKRT 相同性解析による交差性が予測される生物種
Full Name	S100 calcium binding protein A11
Calculated molecular weight	105 aa, 12 kDa
Observed molecular weight	12 kDa
GenBank accession number	BC001410
Gene Symbol	S100A11
Gene ID (NCBI)	6282
RRID	AB_2183478
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P31949
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

S100A11 (also known as S100C or calziggarin), is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs (PMID: 18694925). S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. First discovered in 1989, S100A11 has since been proposed to have direct biological functions in an assortment of physiological processes such as endo- and exocytosis, regulation of enzyme activity, cell growth and regulation, apoptosis and inflammation (PMID: 15241500). Chromosomal rearrangements and altered expression of S100A11 have been implicated in tumor metastasis. This S100A11 antibody (10237-1-AP) has also been instrumental in investigations into S100A11's role in the development of brain tumors in tuberous sclerosis complex (TSC) patients (PMID: 20133820).

Protocols

Product Specific Protocols
FC protocol for S100A11 antibody 10237-1-AP	Download protocol
IF protocol for S100A11 antibody 10237-1-AP	Download protocol
IHC protocol for S100A11 antibody 10237-1-AP	Download protocol
IP protocol for S100A11 antibody 10237-1-AP	Download protocol
WB protocol for S100A11 antibody 10237-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Nat Commun S100A11 is required for efficient plasma membrane repair and survival of invasive cancer cells. Authors - Jyoti K Jaiswal View Article
human	IHC	Metabolism The S100 calcium-binding protein A11 promotes hepatic steatosis through RAGE-mediated AKT-mTOR signaling. Authors - Fei Teng View Article
	WB,IF	Redox Biol Apoptotic breast cancer cells after chemotherapy induce pro-tumour extracellular vesicles via LAP-competent macrophages Authors - Qi Zhang View Article
human	WB	Theranostics Reciprocal interactions between malignant cells and macrophages enhance cancer stemness and M2 polarization in HBV-associated hepatocellular carcinoma Authors - Qingyang Zhang View Article
human	WB	J Clin Invest S100A11 regulates hepatic cholesterol metabolism and promotes steatohepatitis via non-canonical SREBP2 signaling Authors - Mingfeng Zhan View Article
		Cell Death Dis Comparative analysis of S100A10 and S100A11 in MASLD and hepatic cancer development revealed a tumor suppressive role for S100A10 Authors - Etienne Delangre View Article

Validation Data Gallery

WB analysis using 10237-1-AP

WB analysis using 10237-1-AP

WB analysis of PC-3 cells using 10237-1-AP

WB analysis using 10237-1-AP

WB analysis of PC-3 using 10237-1-AP

WB analysis of human lung using 10237-1-AP

WB analysis of HEK-293 using 10237-1-AP

WB analysis of COLO 320 using 10237-1-AP

WB analysis of human heart using 10237-1-AP

WB analysis of BxPC-3 using 10237-1-AP

WB analysis of PC-12 using 10237-1-AP

IP experiment of PC-3 using 10237-1-AP

IP experiment of DU 145 using 10237-1-AP

IHC staining of human cervical cancer using 10237-1-AP

IHC staining of human lung cancer using 10237-1-AP

IHC staining of human thyroid cancer using 10237-1-AP

IHC staining of mouse kidney using 10237-1-AP

IHC staining of rat kidney using 10237-1-AP

IHC staining of human liver cancer using 10237-1-AP

IHC staining of human breast cancer using 10237-1-AP

IHC staining of human breast cancer using 10237-1-AP

IHC staining of human colon using 10237-1-AP

IHC staining of human ovary tumor using 10237-1-AP

IHC staining of human ovary tumor using 10237-1-AP

IHC staining of human colon cancer using 10237-1-AP

IHC staining of human colon cancer using 10237-1-AP

IF Staining of PC-3 using 10237-1-AP

IF Staining of BxPC-3 using 10237-1-AP

IF Staining of BxPC-3 using 10237-1-AP

FC experiment of PC-3 using 10237-1-AP

S100A11 Polyclonal antibody

S100A11 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

S100A11 is required for efficient plasma membrane repair and survival of invasive cancer cells.

The S100 calcium-binding protein A11 promotes hepatic steatosis through RAGE-mediated AKT-mTOR signaling.

Apoptotic breast cancer cells after chemotherapy induce pro-tumour extracellular vesicles via LAP-competent macrophages

Reciprocal interactions between malignant cells and macrophages enhance cancer stemness and M2 polarization in HBV-associated hepatocellular carcinoma

S100A11 regulates hepatic cholesterol metabolism and promotes steatohepatitis via non-canonical SREBP2 signaling

Comparative analysis of S100A10 and S100A11 in MASLD and hepatic cancer development revealed a tumor suppressive role for S100A10

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